camp analog 8 cpt camp Search Results


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BIOLOG Life Science Institute 8-pmeopt-2 o-me-camp
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FUJIFILM l012 (luminol analog, 8-amino-5-chloro-7-phenylpyrido [3,4-d] pyridazine-1,4(2h,3h) dione
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BIOLOG Life Science Institute 8-chlorophenylthio-camp (8-cpt-camp
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
8 Chlorophenylthio Camp (8 Cpt Camp, supplied by BIOLOG Life Science Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pain Therapeutics betulinic acid analog 8
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
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Biomol GmbH cell permeable camp analog 8-bromo-camp
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
Cell Permeable Camp Analog 8 Bromo Camp, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jena Bioscience 8 atp analog
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
8 Atp Analog, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BIOLOG Life Science Institute 8-(4-chlorophenylthio)guanosine-3′,5′-cyclic monophosphate (8-pcpt-cgmp)
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
8 (4 Chlorophenylthio)guanosine 3′,5′ Cyclic Monophosphate (8 Pcpt Cgmp), supplied by BIOLOG Life Science Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tocris camp analog 8 pcpt 2 o me camp am
NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by <t>8-CPT-cAMP.</t> A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.
Camp Analog 8 Pcpt 2 O Me Camp Am, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by 8-CPT-cAMP. A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.

Journal: The Journal of Neuroscience

Article Title: Cocaine-Dependent Acquisition of Locomotor Sensitization and Conditioned Place Preference Requires D1 Dopaminergic Signaling through a Cyclic AMP, NCS-Rapgef2, ERK, and Egr-1/Zif268 Pathway

doi: 10.1523/JNEUROSCI.1497-20.2020

Figure Lengend Snippet: NCS-Rapgef2-dependent phospho-ERK activation and Egr-1 upregulation in neuroendocrine cell line NS-1 induced by 8-CPT-cAMP. A, NS-1 cells (NS-1(Rapgef2+/+)) or NS-1 cells with NCS-Rapgef2 KO by CRISPR (NS-1 (Rapgef2−/−)) were treated with 100 μm 8-CPT-cAMP or 0.01% DMSO (8-CPT-cAMP (–) group) for 1 h; then the cell lysate was collected for Western blot analysis of pan-ERK, phospho-ERK, and Egr-1. Equal amount of total protein (20 µg) was loaded in each lane. B, Phospho-ERK and Egr-1 protein levels were quantified with ImageJ and compared with NS1 (Rapgef2+/+)/8-CPT-cAMP (–) group (n = 3 per group). *p < 0.05; **p < 0.001; post hoc Bonferroni test following two-way ANOVA. Columns and bars represent mean ± sem and scattered points represent individual values. ns, not significant.

Article Snippet: The cell-permeable cAMP analog 8-chlorophenylthio-cAMP (8-CPT-cAMP) was purchased from Biolog Life Science Institute, and the stock solution was prepared at 100 m m in DMSO, diluted in culture media to a final concentration of 100 μ m .

Techniques: Activation Assay, CRISPR, Western Blot